• Test kits are easy to use and are available for several diseases (viruses, fungi and bacteria) important to floriculture crops.
• AgriChek kits  are available from Hydros Inc. to detect fungi Pythium, Rhizoctonia and Phytophthora , bacteria Xanthomonas campestris pv . pelargonii (Xcp) (Bacterial Wilt) and Impatiens Necrotic Spot Virus
• Alert kits from Neogen Corp. are available for Pythium, Phytophthora, and Rhizoctonia.
• Each company has kits that are used a little differently, however the same principal applies. Tests are based on antibodies that are designed to recognize specific antigen (ie. INSV). 
• Any antigen present in the plant sample is extracted by grinding and then mixing with the extraction solution. The extracted antigen is then allowed to react with antibodies that capture specific antigens.
• PCR tests are more sensitive and accurate than ELISA tests and are better suited to plant sample that is likely to have lower numbers of pathogens. 
• In recent years there is emergence of a new concept namely Integrated Pest Management (IPM), which emphasises on prevention and early detection of plant diseases, rather than subsequent control using high value and toxic agro-chemicals.
• Plant diagnostic kit used for this purpose is fast growing in our country looking to the benefits derived from it.
• The Extract-N-Amp Plant PCR Kit offers a novel Extraction Solution that eliminates the need for freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column DNA purification, or alcohol precipitation.
• Agdia offers its QTA-Tospo test kit for the detection of Tomato Spotted Wilt Virus (TSWV) and Impatiens Necrotic Spot Virus (INSV). 
• The QIAGEN Multiplex PCR Kit contains a master mix specifically designed for multiplex PCR applications.
• Mycotoxin test kits work by one of two methods: thin layer chromatography (TLC) or immunochemistry. While the TLC method has been around for a long time, it has both advantages and disadvantages compared to the immunochemical methods.
• One advantage of the TLC method is that more than one mycotoxin can be tested for with a single kit. With the immunochemical kits, a separate kit must be purchased for each mycotoxin.
• A second advantage of the TLC method is that the mycotoxin is actually visualized. The immunochemical kits detect the mycotoxin indirectly and thus are more prone to error. Error can be reduced by purchasing test kits from reputable companies and carefully following the instructions.
• Test kits are available for aflatoxin, zearalenone, ochratoxin, and fumonisin. Price: columns for each test are 57.50-10; each mycotoxin requires a separate test. Other needed materials are sold separately. 

Detection Methods for Virus

• Banana Improvement through Gamma Irradiation And Testing
• Innovative Tools for Detection Of Plant Pathogenic Viruses And Bacteria
• Transgenic Plants Expressing Potato Virus X ORF2 Protein
• RNA Extraction For Detection Of Grapevine Leafroll
• Identification And Detection Of Emerging Viruses
• Detection of Four Apple Viruses

Detection Methods For Bacteria

• Plant Disease Diagnosis
• Detection of Xanthomonas translucens on barley seed
• Evaluation of Pruning as a Method to Reduce Damage by Oleander Leaf Scorch 
• Detection of Intracellular Bacteria in the Buds of Scotch Pine
• Taxonomic Study of Bacteria Isolated from Plants

Detection Methods For Fungi

• Speed detection of pests in China
• Detection of entomopathogenic fungi in peridomestic triatomine
• Detection and quantification of toxigenic Fusarium fungi 
• Tall Fescue Endophyte Detection
• Detection and Identification of Fungi from Fungus Balls

Detection Methods for Nematodes

• Detection and characterization of Pasteuria 16S rRNA gene sequences from nematodes and soils
• Influence of Cover Crop Rotation and Conventional Practices on Grapevine Plant Growth and Weed Growth
• Reverse transcription polymerase chain reaction
• Dehydration-Specific Induction of Hydrophilic Protein Genes in the Anhydrobiotic Nematode
• Nematode Assay Laboratory
• Leather Jackets
• Plant gene expression associated with susceptibility to nematodes

Detection Of Insects and Phytoplasma

• Protocol For the Diagnosis Of Quarantine Organisms
• Pea Leafminer
• Thrips Palmi Karny
• Detection Of Aster Yellows Group Phytoplasma
• Detection And Identification Of A Phytoplasma From Lucerne
• Detection And Identification Of Phytoplasma DNA In Symptomatic Mushrooms
• Different Phytoplasma DNA Extraction Methods
• Improved Detection Methods for Fruit Tree 
• Molecular Detection And Characterization Of Phytoplasma

Products

• Product Range: Kits List
• Tospo Test Kit
• Bioreba
• Development Of Potato Virus On-site Test Kits
• Seeplex TV4 Detection Kit
• Biotech Products
• Molecular Biology Kits
• Qiagen Multiplex PCR And DNeasy Kits
• Diagnostic Test Kits
• Phosphatase Detection Kit
• Extract-N-Amp Plant PCR Kits
• FF MicroPlate
• Mycotoxin Test Kits
• RNeasy Plant Mini Kit
• Fungi Fluor Kit
• Termite And Insect Pest Detection Kit
• Methods for Detection of Transgenic Plants

• New Technologies for the detection and identification of Pathogens,  pests And Environmental Pollutants
• Taiwan's Development Center For Biotechnology
• Premium Seed Treatments
• New Pathogen Testing Technologies
• On-Site Tests For Fungi And Viruses In Nurseries And Greenhouses
• RT-PCR Detection of Seedborne Cowpea aphid-borne mosaic virus in Peanut
• Getting Technologies Out To Industry
• Diagnosis and Control of Phytophthora Diseases
• Control of plant pathogenic fungus Rhizoctonia solani by microorganisms
• Techniques for using the AED-2000L
• Cloning and Sequencing of Phytoplasma Ribosomal DNA

Consultants

• Earthcare Technology & Consulting services
• Steven Nadler
• Tasmanian Institute Of Agricultural Research
• Wageningen UR
• Dr.Ulrich Commandeur
• Charles P.Woloshuk 

Companies Involved

• Diagnostic, Testing Kits & Analytical Services- Companies Lists
• Agdia
• Bioreba AG
• Eiken Chemical Co Ltd
• Pocket Diagnostic
• Seegene Inc.,
• Hydros Inc.
• Beijing Science Trade Co Ltd
• Diagnostic Test Manufacturers
• Gibberellic Acid Kits And Suppliers
• PG Biotech

Projects

• Manufacturing Unit for Plant Diagnostic Kits
• DNA Program kit
• Standard test kits incorporating novel antibody fusion proteins to detect harmful viruses
• Testing Plant For Virus Infections
• Fungi as Biosensors
• Project ideas using mushrooms and fungi
• Project Work Plan
• Cluster Project

Market And Report

• Gujarat: Biotechnology
• Development of Diagnostic Reagents in the Healthcare Industry
• Custom and library siRNA for efficient gene silencing 
• Annual Report 2005
• Nematode Biodiversity and Systematics
• Detection Of Viruses In Verbena 
• Highly sensitive PCR detection kits for the efficient multiplex detection of viruses
• Fight against Viruses
• Detection of Prunus necrotic ringspot virus in begonia by RT-PCR
• National Program Annual Report
• Environmental Toxicology Newsletter
• Functional Expression Of A Single- Chain Antibody
• Reports On Plant Diseases
• Scientists develop better way to detect presence of soybean fungus